Biotechnology And Its Applications Mock Tests

28 questions available

Biotechnology And Its Applications Mock Test 1

Questions: 28

Sample Questions

Class 12 Biology

A cloning vector must contain an "origin of replication" (ori) sequence because this element:

A Allows the plasmid to replicate independently within the host cell, ensuring the inserted gene is copied along with the vector

B Provides resistance to antibiotics, enabling selection of transformed cells

C Contains multiple restriction enzyme sites for inserting foreign DNA

D Encodes the lacZ gene used for blue-white screening of recombinants

Class 12 Biology

In gene therapy for ADA (Adenosine Deaminase) deficiency, the most commonly used approach involves:

A Inserting a functional ADA cDNA into lymphocytes using a retroviral vector

B Using CRISPR-Cas9 to directly edit the ADA gene in somatic cells

C Administering regular injections of purified ADA enzyme

D Transplanting bone marrow from a fully HLA-matched sibling donor

Class 12 Biology

Transgenic animals are produced for various purposes. Which of the following is NOT a valid application of transgenic animals:

A Production of human proteins (e.g., alpha-1-antitrypsin in transgenic sheep) for treating genetic diseases

B Testing the safety of vaccines, such as the polio vaccine using transgenic mice

C Creating clones of endangered species for in-situ conservation

D Understanding gene action and the development of diseases like cancer

Class 12 Biology

Gene therapy for ADA deficiency was first clinically tried in which year?

A 1990

B 1995

C 2000

D 2005

Class 12 Biology

Gene therapy for ADA (adenosine deaminase) deficiency involves:

A Permanent insertion of the functional ADA gene into every cell of the patient's body at birth

B Introduction of lymphocytes carrying the functional ADA gene using a retroviral vector, requiring periodic administration

C Replacement of the defective bone marrow with that of a matched donor without any gene modification

D Administration of the ADA enzyme directly into the bloodstream as a replacement therapy

Class 12 Biology

Protoplast fusion (somatic hybridisation) is used to:

A Combine desirable traits from two different species that cannot be crossed sexually, producing a somatic hybrid

B Produce seedless fruits by treating unfertilised ovaries with plant growth hormones

C Generate transgenic plants by inserting a single gene using Agrobacterium-mediated transformation

D Induce mutations in plant cells using chemical mutagens like EMS or radiation

Class 12 Biology

Transgenic animals are primarily used for all of the following purposes EXCEPT:

A Direct production of bulk electricity through bioreactor systems that convert metabolic energy into electrical current

B Modeling human diseases, studying gene function, testing vaccine safety, and producing therapeutic proteins

C Understanding gene regulation and developmental biology by observing effects of gene overexpression or knockout

D Improving livestock traits such as growth rate, feed efficiency, and disease resistance

Class 12 Biology

Human insulin is produced commercially using recombinant DNA technology in E. coli. The process involves:

A Producing the A and B chains of insulin separately in E. coli, purifying them, and then joining them by disulphide bond formation

B Inserting the complete human insulin gene into E. coli, which then produces active insulin directly

C Extracting insulin from the pancreas of slaughtered animals and chemically modifying it to match human insulin

D Using yeast cells to produce insulin without any genetic modification

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