Biotechnology And Its Applications Mock Tests

28 questions available

Biotechnology And Its Applications Mock Test 1

Questions: 28

Sample Questions

Class 12 Biology

Transgenic animals are produced for various purposes. Which of the following is NOT a valid application of transgenic animals:

A Production of human proteins (e.g., alpha-1-antitrypsin in transgenic sheep) for treating genetic diseases

B Testing the safety of vaccines, such as the polio vaccine using transgenic mice

C Creating clones of endangered species for in-situ conservation

D Understanding gene action and the development of diseases like cancer

Class 12 Biology

Gene therapy for adenosine deaminase (ADA) deficiency involves which of the following approaches?

A Permanent insertion of the functional ADA gene into the patient's somatic cells during infancy

B Periodic infusion of genetically engineered lymphocytes expressing functional ADA

C Administration of ADA enzyme replacement therapy via intravenous injection

D Use of CRISPR-Cas9 to edit the defective ADA gene in the patient's germline cells

Class 12 Biology

Adeno-associated virus (AAV) vectors are preferred in clinical gene therapy over retroviral vectors because AAV:

A Integrates primarily as episomal DNA (non-integrating), reducing the risk of insertional mutagenesis while providing long-term gene expression

B Can carry a much larger cargo capacity (>20 kb) compared to all other viral vectors

C Does not require any host cell machinery for replication or gene expression

D Is non-pathogenic to humans and cannot elicit any immune response whatsoever

Class 12 Biology

Transgenic animals are primarily used for all of the following purposes EXCEPT:

A Direct production of bulk electricity through bioreactor systems that convert metabolic energy into electrical current

B Modeling human diseases, studying gene function, testing vaccine safety, and producing therapeutic proteins

C Understanding gene regulation and developmental biology by observing effects of gene overexpression or knockout

D Improving livestock traits such as growth rate, feed efficiency, and disease resistance

Class 12 Biology

Protoplast fusion (somatic hybridisation) is used to:

A Combine desirable traits from two different species that cannot be crossed sexually, producing a somatic hybrid

B Produce seedless fruits by treating unfertilised ovaries with plant growth hormones

C Generate transgenic plants by inserting a single gene using Agrobacterium-mediated transformation

D Induce mutations in plant cells using chemical mutagens like EMS or radiation

Class 12 Biology

A cloning vector must contain an "origin of replication" (ori) sequence because this element:

A Allows the plasmid to replicate independently within the host cell, ensuring the inserted gene is copied along with the vector

B Provides resistance to antibiotics, enabling selection of transformed cells

C Contains multiple restriction enzyme sites for inserting foreign DNA

D Encodes the lacZ gene used for blue-white screening of recombinants

Class 12 Biology

Bt cotton is resistant to which type of pest:

A Root-knot nematodes

B Bollworms (Helicoverpa armigera)

C Aphids

D Whiteflies

Class 12 Biology

Stem cells are defined by their unique ability to:

A Undergo self-renewal and differentiate into specialized cell types

B Produce energy through anaerobic respiration more efficiently than other cells

C Form the structural framework of connective tissues only

D Migrate through the bloodstream to sites of injury exclusively

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